Molecular characteristics of chicken liver arginase
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چکیده
منابع مشابه
Molecular characteristics of rat liver arginase.
The molecular weight of approximately 1,500-fold purified arginase from rat liver (specific activity = 19,500 pmoles of urea per min at 25’ per mg of protein nitrogen) was determined by the method of sedimentation equilibrium to be 118,000. The sedimentation velocity constant is 6.1 S, the diffusion coefficient 5.2 X 10U7 cm2 per sec. Alteration in pH, removal of Mn2+, and replacement of Mn2+ b...
متن کاملMolecular Characteristics of Chicken Liver Arginase By ENRICO GRAZI and ERMES MAGRI
A purification procedure for the preparation of chicken liver arginase in a homogeneous form is presented. The enzyme hydrolyses both arginine and argininic acid. Kinetic analysis reveals that the enzyme binds arginine when the amino group is protonated or unprotonated; however, the unprotonated form seems to be hydrolysed more rapidly. The enzyme exchanges with the medium approx. 1.6Mn2+ ions ...
متن کاملMolecular charcteristics of chicken kidney arginase.
Chicken kidney contains two arginases with different sedimentation coefficients and substrate specificity. The ligher of these arginases, which hydrolyses only L-arginine, has been purified about 3000-fold. Like the "ureotelic" arginase, developed in chicken liver after starvation, it displays many of the properties of the arginase of the "ureotelic" species. This seems to exclude the possibili...
متن کاملOn molecular sizes of rat liver arginase.
Arginase exists as 2 or more molecular species in the size profile of the soluble macromolecules of rat liver in the absence of added Mn@. The principal species is that of approximately 120,000 molecular weight, as previously re ported by others. In addition, after activation by Mn@ , there are detectable small but significant amounts of arginase of about 30,000—40,000 molecular weight, presu...
متن کاملArginase isoenzymes in human cirrhotic liver.
Cirrhosis leads to an inability of the liver to perform its biochemical functions. It can also lead to hepatocellular carcinoma in which, as we showed lately, arginase isoenzyme pattern changes. The present work presents our results on arginase isoenzymes and their possible role in liver cirrhosis. The study was performed on tissues obtained during liver transplantation from 60 patients with li...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1972
ISSN: 0306-3283
DOI: 10.1042/bj1260667